› 2f7f

X-ray diffraction
2Å resolution

Crystal structure of Enterococcus faecalis putative nicotinate phosphoribosyltransferase, NEW YORK STRUCTURAL GENOMICS CONSORTIUM

Released:
DOI: 10.2210/pdb2f7f/pdb
PDB entry 2f7f coloured by chain, front view.
PDB entry 2f7f coloured by chain, side view.
PDB entry 2f7f coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry authors: Patskovsky Y, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H(2)O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182763 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinate phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 494 amino acids
Theoretical weight: 56.6 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q830Y8 (Residues: 1-494; Coverage: 100%)
Gene name: EF_2626
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 8 x SO4
Ligand NIO 2 x NIO
Ligand DPO 1 x DPO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I222
Unit cell:
a: 71.325Å b: 110.627Å c: 154.189Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.171 0.214
Expression system: Escherichia coli BL21(DE3)

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