› 2f7s

X-ray diffraction
2.7Å resolution

The crystal structure of human Rab27b bound to GDP

Released:
DOI: 10.2210/pdb2f7s/pdb
PDB entry 2f7s coloured by chain, front view.
PDB entry 2f7s coloured by chain, side view.
PDB entry 2f7s coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Wang J, Ismail S, Shen Y, Tempel W, Arrowsmith CH, Edwards AM, Sundstrom M, Bochkarev A, Park HW, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-27B Chains: A, B
Molecule details ›
Chains: A, B
Length: 217 amino acids
Theoretical weight: 24.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00194 (Residues: 4-201; Coverage: 91%)
Gene name: RAB27B
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 55.469Å b: 64.023Å c: 153.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.245 0.292
Expression system: Escherichia coli

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