› 2f8f

X-ray diffraction
2.1Å resolution

Crystal structure of the Y10F mutant of the gluathione s-transferase from schistosoma haematobium

Released:
DOI: 10.2210/pdb2f8f/pdb
PDB entry 2f8f coloured by chain, front view.
PDB entry 2f8f coloured by chain, side view.
PDB entry 2f8f coloured by chain, top view.
Source organism: Schistosoma haematobium
Primary publication:
Probing the mechanism of GSH activation in Schistosoma haematobium glutathione-S-transferase by site-directed mutagenesis and X-ray crystallography.
Baiocco P, Gourlay LJ, Angelucci F, Fontaine J, Hervé M, Miele AE, Trottein F, Brunori M, Bellelli A
J Mol Biol 360 678-89 (2006)
PMID: 16777141

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase class-mu 28 kDa isozyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.92 KDa
Source organism: Schistosoma haematobium
Expression system: Escherichia coli
UniProt:
  • Canonical: P30113 (Residues: 1-211; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 53.227Å b: 77.594Å c: 53.143Å
α: 90° β: 94.26° γ: 90°
R-values:
R R work R free
0.224 0.221 0.288
Expression system: Escherichia coli

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Citations

3 review citations

Glutathione transferases from parasites: a biochemical view.
Torres-Rivera et al. (2008)
2 more

1 mention without citation

Chromatographic and computational studies of ligands associated with bilharziasis.
Abubakar et al. (2019)