2f8k

X-ray diffraction
2Å resolution

Sequence specific recognition of RNA hairpins by the SAM domain of Vts1

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Sequence-specific recognition of RNA hairpins by the SAM domain of Vts1p.
Nat Struct Mol Biol 13 168-76 (2006)
PMID: 16429151

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-170700 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-binding protein VTS1 Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 10.08 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q08831 (Residues: 436-523; Coverage: 17%)
Gene names: VTS1, YOR359W
Sequence domains: SAM domain (Sterile alpha motif)
Structure domains: Transcription Factor, Ets-1
5'-R(*UP*AP*AP*UP*CP*UP*UP*UP*GP*AP*CP*AP*GP*AP*UP*U)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 5.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002
Spacegroup: P212121
Unit cell:
a: 27.55Å b: 44.53Å c: 129.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.277
Expression systems:
  • Escherichia coli BL21
  • Not provided