› 2f96

X-ray diffraction
2.09Å resolution

2.1 A crystal structure of Pseudomonas aeruginosa rnase T (Ribonuclease T)

Released:
DOI: 10.2210/pdb2f96/pdb
PDB entry 2f96 coloured by chain, front view.
PDB entry 2f96 coloured by chain, side view.
PDB entry 2f96 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Crystal structure of RNase T, an exoribonuclease involved in tRNA maturation and end turnover.
Zuo Y, Zheng H, Wang Y, Chruszcz M, Cymborowski M, Skarina T, Savchenko A, Malhotra A, Minor W
Structure 15 417-28 (2007)
PMID: 17437714

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191141 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease T Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 25.08 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HY82 (Residues: 1-224; Coverage: 100%)
Gene names: PA3528, rnt
Sequence domains: Exonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 49.879Å b: 76.636Å c: 61.666Å
α: 90° β: 93.55° γ: 90°
R-values:
R R work R free
0.16 0.158 0.204
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The critical role of RNA processing and degradation in the control of gene expression.
Arraiano et al. (2010)
2 more

6 mentions without citation

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
5 more