› 2f9h

X-ray diffraction
1.57Å resolution

The Crystal Structure of PTS System IIA Component from Enterococcus faecalis V583

Released:
DOI: 10.2210/pdb2f9h/pdb
PDB entry 2f9h coloured by chain, front view.
PDB entry 2f9h coloured by chain, side view.
PDB entry 2f9h coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry authors: Kim Y, Quartey P, Moy S, Bargassa M, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182765 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system, IIA component Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.19 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q831B0 (Residues: 1-126; Coverage: 100%)
Gene name: EF_2603
Sequence domains: PTS system glucitol/sorbitol-specific IIA component
Structure domains: Phosphotransferase system, glucitol/sorbitol-specific IIA component

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM, APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 58.249Å b: 58.249Å c: 140.443Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.169 0.229
Expression system: Escherichia coli BL21(DE3)

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