› 2f9s

X-ray diffraction
1.4Å resolution

2nd Crystal Structure Of A Soluble Domain Of ResA In The Oxidised Form

Released:
DOI: 10.2210/pdb2f9s/pdb
PDB entry 2f9s coloured by chain, front view.
PDB entry 2f9s coloured by chain, side view.
PDB entry 2f9s coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Mechanism of substrate specificity in Bacillus subtilis ResA, a thioredoxin-like protein involved in cytochrome c maturation.
Colbert CL, Wu Q, Erbel PJ, Gardner KH, Deisenhofer J
Proc Natl Acad Sci U S A 103 4410-5 (2006)
PMID: 16537372

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152807 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol-disulfide oxidoreductase ResA Chains: A, B
Molecule details ›
Chains: A, B
Length: 151 amino acids
Theoretical weight: 17.25 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P35160 (Residues: 37-179; Coverage: 80%)
Gene names: BSU23150, resA, ypxA
Sequence domains: AhpC/TSA family
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P65
Unit cell:
a: 61.186Å b: 61.186Å c: 166.999Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.162 0.175
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Cytochrome c biogenesis: the Ccm system.
Sanders et al. (2010)
4 more

3 mentions without citation

Protein Machineries Involved in the Attachment of Heme to Cytochrome c: Protein Structures and Molecular Mechanisms.
Travaglini-Allocatelli C. (2013)
2 more