› 2fbo

X-ray diffraction
1.85Å resolution

Crystal Structure of the Two Tandem V-type Regions of VCBP3 (v-region-containing chitin binding protein) to 1.85 A

Released:
DOI: 10.2210/pdb2fbo/pdb
PDB entry 2fbo coloured by chain, front view.
PDB entry 2fbo coloured by chain, side view.
PDB entry 2fbo coloured by chain, top view.
Source organism: Branchiostoma floridae
Primary publication:
Ancient evolutionary origin of diversified variable regions demonstrated by crystal structures of an immune-type receptor in amphioxus.
Hernández Prada JA, Haire RN, Allaire M, Jakoncic J, Stojanoff V, Cannon JP, Litman GW, Ostrov DA
Nat Immunol 7 875-82 (2006)
PMID: 16799561

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184726 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
chitinase Chain: J
Molecule details ›
Chain: J
Length: 250 amino acids
Theoretical weight: 27.29 KDa
Source organism: Branchiostoma floridae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8I9N0 (Residues: 18-267; Coverage: 79%)
Gene name: VCBP3
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P61
Unit cell:
a: 109.597Å b: 109.597Å c: 48.845Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.228 0.269
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Origin and evolution of the adaptive immune system: genetic events and selective pressures.
Flajnik et al. (2010)
14 more

2 mentions without citation

Ancient evolutionary origin of diversified variable regions demonstrated by crystal structures of an immune-type receptor in amphioxus.
Hernández Prada et al. (2006)
1 more