› 2fbt

X-ray diffraction
2.05Å resolution

WRN exonuclease

Released:
DOI: 10.2210/pdb2fbt/pdb
PDB entry 2fbt coloured by chain, front view.
PDB entry 2fbt coloured by chain, side view.
PDB entry 2fbt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
WRN exonuclease structure and molecular mechanism imply an editing role in DNA end processing.
Perry JJ, Yannone SM, Holden LG, Hitomi C, Asaithamby A, Han S, Cooper PK, Chen DJ, Tainer JA
Nat Struct Mol Biol 13 414-22 (2006)
PMID: 16622405

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 23.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14191 (Residues: 38-236; Coverage: 14%)
Gene names: RECQ3, RECQL2, WRN
Sequence domains: 3'-5' exonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

1 bound ligand:
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3221
Unit cell:
a: 80.269Å b: 80.269Å c: 93.23Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.209 0.241
Expression system: Escherichia coli

Quick links

Citations

38 review citations

Mechanisms of DNA damage, repair, and mutagenesis.
Chatterjee et al. (2017)
37 more

6 mentions without citation

The RNase H-like superfamily: new members, comparative structural analysis and evolutionary classification.
Majorek et al. (2014)
5 more