› 2fca

X-ray diffraction
2.1Å resolution

The structure of BsTrmB

Released:
DOI: 10.2210/pdb2fca/pdb
PDB entry 2fca coloured by chain, front view.
PDB entry 2fca coloured by chain, side view.
PDB entry 2fca coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase.
Zegers I, Gigot D, van Vliet F, Tricot C, Aymerich S, Bujnicki JM, Kosinski J, Droogmans L
Nucleic Acids Res 34 1925-34 (2006)
PMID: 16600901

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (guanine-N(7)-)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 213 amino acids
Theoretical weight: 24.65 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O34522 (Residues: 1-213; Coverage: 100%)
Gene names: BSU29900, trmB, ytmQ
Sequence domains: Putative methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: R3
Unit cell:
a: 178.83Å b: 178.83Å c: 41.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.202 0.233
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Methylated nucleosides in tRNA and tRNA methyltransferases.
Hori H. (2014)
8 more

6 mentions without citation

7-Methylguanosine Modifications in Transfer RNA (tRNA).
Tomikawa C. (2018)
5 more