› 2fcj

X-ray diffraction
1.3Å resolution

Structure of small TOPRIM domain protein from Bacillus stearothermophilus.

Released:
DOI: 10.2210/pdb2fcj/pdb
PDB entry 2fcj coloured by chain, front view.
PDB entry 2fcj coloured by chain, side view.
PDB entry 2fcj coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structure and putative function of small Toprim domain-containing protein from Bacillus stearothermophilus.
Rezácová P, Borek D, Moy SF, Joachimiak A, Otwinowski Z
Proteins 70 311-9 (2008)
PMID: 17705269

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Toprim domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 119 amino acids
Theoretical weight: 13.72 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5KVJ9 (Residues: 1-119; Coverage: 100%)
Gene name: GK3002
Sequence domains: Toprim domain
Structure domains: Dna Topoisomerase Vi A Subunit; Chain: A, domain 2

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 2 x SO4
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 36.617Å b: 72.72Å c: 116.299Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.214
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Topoisomerases and site-specific recombinases: similarities in structure and mechanism.
Yang W. (2010)
15 other articles

2 mentions without citation

Crystal structure and putative function of small Toprim domain-containing protein from Bacillus stearothermophilus.
Rezácová et al. (2008)
1 more