› 2fcn

X-ray diffraction
2.2Å resolution

X-ray Crystal Structure of a Chemically Synthesized [D-Val35]Ubiquitin with a Cubic Space Group

Released:
DOI: 10.2210/pdb2fcn/pdb
PDB entry 2fcn coloured by chain, front view.
PDB entry 2fcn coloured by chain, side view.
PDB entry 2fcn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dissecting the energetics of protein alpha-helix C-cap termination through chemical protein synthesis.
Bang D, Gribenko AV, Tereshko V, Kossiakoff AA, Kent SB, Makhatadze GI
Nat Chem Biol 2 139-43 (2006)
PMID: 16446709

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B
Molecule details ›
Chains: A, B
Length: 76 amino acids
Theoretical weight: 8.6 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand CD 12 x CD
Ligand ACT 3 x ACT
1 modified residue:
Ligand DVA 2 x DVA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P4332
Unit cell:
a: 106.152Å b: 106.152Å c: 106.152Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.231 0.278
Expression system: Not provided

Quick links

Citations

7 review citations

Total chemical synthesis of proteins.
Kent SB. (2009)
6 more

4 mentions without citation

Analysis of folded structure and folding thermodynamics in heterogeneous-backbone proteomimetics.
Santhouse et al. (2021)
3 more