› 2fdc

X-ray diffraction
3.3Å resolution

Structural Basis of DNA Damage Recognition and Processing by UvrB: crystal structure of a UvrB/DNA complex

Released:
DOI: 10.2210/pdb2fdc/pdb
PDB entry 2fdc coloured by chain, front view.
PDB entry 2fdc coloured by chain, side view.
PDB entry 2fdc coloured by chain, top view.
Source organism: [Bacillus] caldotenax
Primary publication:
Structural basis for DNA recognition and processing by UvrB.
Truglio JJ, Karakas E, Rhau B, Wang H, DellaVecchia MJ, Van Houten B, Kisker C
Nat Struct Mol Biol 13 360-4 (2006)
PMID: 16532007

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
UvrABC system protein B Chains: A, B
Molecule details ›
Chains: A, B
Length: 658 amino acids
Theoretical weight: 75.59 KDa
Source organism: [Bacillus] caldotenax
Expression system: Escherichia coli
UniProt:
  • Canonical: P56981 (Residues: 2-190, 191-658; Coverage: 100%)
Gene name: uvrB
Sequence domains:
Structure domains:
5'-D(P*CP*GP*GP*CP*TP*CP*CP*AP*TP*CP*TP*CP*TP*AP*CP*CP*GP*CP*AP*A)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 20 nucleotides
Theoretical weight: 6.01 KDa

Ligands and Environments

1 bound ligand:
Ligand FLQ 1 x FLQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P3221
Unit cell:
a: 153.265Å b: 153.265Å c: 160.17Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.258
Expression system: Escherichia coli

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Citations

12 review citations

Eukaryotic nucleotide excision repair: from understanding mechanisms to influencing biology.
Shuck et al. (2008)
11 more

19 mentions without citation

Prokaryotic nucleotide excision repair.
Kisker et al. (2013)
18 more