› 2fe3

X-ray diffraction
1.75Å resolution

The crystal structure of bacillus subtilis PerR-Zn reveals a novel Zn(Cys)4 Structural redox switch

Released:
DOI: 10.2210/pdb2fe3/pdb
PDB entry 2fe3 coloured by chain, front view.
PDB entry 2fe3 coloured by chain, side view.
PDB entry 2fe3 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of the apo-PerR-Zn protein from Bacillus subtilis.
Traoré DA, El Ghazouani A, Ilango S, Dupuy J, Jacquamet L, Ferrer JL, Caux-Thang C, Duarte V, Latour JM
Mol Microbiol 61 1211-9 (2006)
PMID: 16925555

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxide operon regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.45 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P71086 (Residues: 1-145; Coverage: 100%)
Gene names: BSU08730, perR, ygaG
Sequence domains: Ferric uptake regulator family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P1
Unit cell:
a: 41.29Å b: 42.16Å c: 53.49Å
α: 82.18° β: 80.11° γ: 60.96°
R-values:
R R work R free
0.172 0.172 0.229
Expression system: Escherichia coli BL21(DE3)

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Citations

16 review citations

Hydrogen peroxide sensing, signaling and regulation of transcription factors.
Marinho et al. (2014)
15 more

10 mentions without citation

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
9 more