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2fen

X-ray diffraction
2.6Å resolution

3-carboxy-cis,cis-muconate lactonizing enzyme from Agrobacterium radiobacter S2

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
2-(carboxymethyl)-5-oxo-2,5-dihydro-2-furoate = 3-carboxy-cis,cis-muconate + H(+).
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-173623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-carboxy-cis,cis-muconate cycloisomerase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 359 amino acids
Theoretical weight: 38.39 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2HNZ1 (Residues: 1-353; Coverage: 100%)
Gene name: pcaB1
Sequence domains: Lyase
Structure domains: Fumarase/aspartase (Central domain)

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 94.03Å b: 205.32Å c: 235.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.2 0.262
Expression system: Escherichia coli