› 2ffi

X-ray diffraction
2.61Å resolution

Crystal Structure of Putative 2-Pyrone-4,6-Dicarboxylic Acid Hydrolase from Pseudomonas putida, Northeast Structural Genomics Target PpR23.

Released:
DOI: 10.2210/pdb2ffi/pdb
PDB entry 2ffi coloured by chain, front view.
PDB entry 2ffi coloured by chain, side view.
PDB entry 2ffi coloured by chain, top view.
Source organism: Pseudomonas putida KT2440
Entry authors: Forouhar F, Su M, Jayaraman S, Conover K, Xiao R, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
2-oxo-2H-pyran-4,6-dicarboxylate + H(2)O = (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 288 amino acids
Theoretical weight: 32.43 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli
UniProt:
  • Canonical: Q88M75 (Residues: 1-280; Coverage: 100%)
Gene name: PP_1699
Sequence domains: Amidohydrolase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P1
Unit cell:
a: 43.392Å b: 46.289Å c: 64.078Å
α: 80.86° β: 76.86° γ: 80.61°
R-values:
R R work R free
0.23 0.228 0.287
Expression system: Escherichia coli

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