› 2ffw

Solution NMR

Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING

Released:
DOI: 10.2210/pdb2ffw/pdb
PDB entry 2ffw coloured by chain, ensemble of 13 models, front view.
PDB entry 2ffw coloured by chain, ensemble of 13 models, side view.
PDB entry 2ffw coloured by chain, ensemble of 13 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING.
Massiah MA, Simmons BN, Short KM, Cox TC
J Mol Biol 358 532-45 (2006)
PMID: 16529770

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Midline-1 Chain: A
Molecule details ›
Chain: A
Length: 78 amino acids
Theoretical weight: 8.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15344 (Residues: 87-164; Coverage: 12%)
Gene names: FXY, MID1, RNF59, TRIM18, XPRF
Structure domains: 30s Ribosomal Protein S14; Chain N

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

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Citations

21 review citations

The control of viral infection by tripartite motif proteins and cyclophilin A.
Towers GJ. (2007)
20 more

5 mentions without citation

In Silico Evaluation of the Effectivity of Approved Protease Inhibitors against the Main Protease of the Novel SARS-CoV-2 Virus.
Eleftheriou et al. (2020)
4 more