› 2fgc

X-ray diffraction
2.3Å resolution

Crystal structure of Acetolactate synthase- small subunit from Thermotoga maritima

Released:
DOI: 10.2210/pdb2fgc/pdb
PDB entry 2fgc coloured by chain, front view.
PDB entry 2fgc coloured by chain, side view.
PDB entry 2fgc coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit.
Petkowski JJ, Chruszcz M, Zimmerman MD, Zheng H, Skarina T, Onopriyenko O, Cymborowski MT, Koclega KD, Savchenko A, Edwards A, Minor W
Protein Sci 16 1360-7 (2007)
PMID: 17586771

Function and Biology Details

Reaction catalysed: 
2 pyruvate = 2-acetolactate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetolactate synthase small subunit Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 22.37 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZ19 (Residues: 1-171; Coverage: 100%)
Gene name: TM_0549
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I422
Unit cell:
a: 104.782Å b: 104.782Å c: 78.511Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.168 0.236
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure and mechanism of inhibition of plant acetohydroxyacid synthase.
Duggleby et al. (2008)
2 more

1 mention without citation

Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit.
Petkowski et al. (2007)