› 2fge

X-ray diffraction
2.1Å resolution

Crystal structure of presequence protease PreP from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb2fge/pdb
PDB entry 2fge coloured by chain, front view.
PDB entry 2fge coloured by chain, side view.
PDB entry 2fge coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The closed structure of presequence protease PreP forms a unique 10,000 Angstroms3 chamber for proteolysis.
Johnson KA, Bhushan S, Ståhl A, Hallberg BM, Frohn A, Glaser E, Eneqvist T
EMBO J 25 1977-86 (2006)
PMID: 16601675

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162699 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Presequence protease 1, chloroplastic/mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 995 amino acids
Theoretical weight: 112.54 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9LJL3 (Residues: 86-1080; Coverage: 92%)
Gene names: At3g19170, MVI11.6, MVI11.7, MVI11_8, PREP1, ZNMP1
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
nonspecific peptide AALTRA Chains: D, E
Molecule details ›
Chains: D, E
Length: 6 amino acids
Theoretical weight: 603 Da
Source organism: Arabidopsis thaliana
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand MG 4 x MG
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 110.834Å b: 114.332Å c: 162.984Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.256
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

21 review citations

Targeting of nucleus-encoded proteins to chloroplasts in plants.
Jarvis P. (2008)
20 more

6 mentions without citation

Amyloid beta-degrading cryptidases: insulin degrading enzyme, presequence peptidase, and neprilysin.
Malito et al. (2008)
5 more