› 2fgq

X-ray diffraction
1.45Å resolution

High resolution X-ray structure of Omp32 in complex with malate

Released:
DOI: 10.2210/pdb2fgq/pdb
PDB entry 2fgq coloured by chain, front view.
PDB entry 2fgq coloured by chain, side view.
PDB entry 2fgq coloured by chain, top view.
Source organism: Delftia acidovorans
Primary publication:
High resolution crystal structures and molecular dynamics studies reveal substrate binding in the porin Omp32.
Zachariae U, Klühspies T, De S, Engelhardt H, Zeth K
J Biol Chem 281 7413-20 (2006)
PMID: 16434398

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane porin protein 32 Chain: X
Molecule details ›
Chain: X
Length: 332 amino acids
Theoretical weight: 34.85 KDa
Source organism: Delftia acidovorans
UniProt:
  • Canonical: P24305 (Residues: 20-351; Coverage: 100%)
Gene name: omp32
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

4 bound ligands:
Ligand BOG 1 x BOG
Ligand CA 3 x CA
Ligand SO4 2 x SO4
Ligand MLT 1 x MLT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P63
Unit cell:
a: 106.21Å b: 106.21Å c: 93.21Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.15 0.149 0.171

Quick links

Citations

4 review citations

Microbe-host interactions: structure and role of Gram-negative bacterial porins.
Galdiero et al. (2012)
3 more

7 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
6 more