› 2fgy

X-ray diffraction
2.2Å resolution

Beta Carbonic Anhydrase from the Carboxysomal Shell of Halothiobacillus neapolitanus (CsoSCA)

Released:
DOI: 10.2210/pdb2fgy/pdb
PDB entry 2fgy coloured by chain, front view.
PDB entry 2fgy coloured by chain, side view.
PDB entry 2fgy coloured by chain, top view.
Source organism: Halothiobacillus neapolitanus
Primary publication:
The structure of beta-carbonic anhydrase from the carboxysomal shell reveals a distinct subclass with one active site for the price of two.
Sawaya MR, Cannon GC, Heinhorst S, Tanaka S, Williams EB, Yeates TO, Kerfeld CA
J Biol Chem 281 7546-55 (2006)
PMID: 16407248

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxysome shell carbonic anhydrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 542 amino acids
Theoretical weight: 60.72 KDa
Source organism: Halothiobacillus neapolitanus
Expression system: Escherichia coli
UniProt:
  • Canonical: O85042 (Residues: 1-514; Coverage: 100%)
Gene names: Hneap_0919, ORF1, csoS3
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P1
Unit cell:
a: 47.738Å b: 77.636Å c: 82.714Å
α: 66.84° β: 78.82° γ: 79.17°
R-values:
R R work R free
0.171 0.169 0.217
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Ecological genomics of marine picocyanobacteria.
Scanlan et al. (2009)
22 more

6 mentions without citation

Atypical Carboxysome Loci: JEEPs or Junk?
USF Genomics Class et al. (2022)
5 more