› 2fh6

X-ray diffraction
1.8Å resolution

Crystal Structure Analysis of Klebsiella pneumoniae pullulanase complexed with glucose

Released:
DOI: 10.2210/pdb2fh6/pdb
PDB entry 2fh6 coloured by chain, front view.
PDB entry 2fh6 coloured by chain, side view.
PDB entry 2fh6 coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Crystal structure of pullulanase: evidence for parallel binding of oligosaccharides in the active site.
Mikami B, Iwamoto H, Malle D, Yoon HJ, Demirkan-Sarikaya E, Mezaki Y, Katsuya Y
J Mol Biol 359 690-707 (2006)
PMID: 16650854

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

2 bound ligands:
Ligand GLC 1 x GLC
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 149.263Å b: 60.42Å c: 133.65Å
α: 90° β: 111.98° γ: 90°
R-values:
R R work R free
0.185 0.185 0.212

Quick links

Citations

10 review citations

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
9 more

1 mention without citation

Structure of the starch-debranching enzyme barley limit dextrinase reveals homology of the N-terminal domain to CBM21.
Møller et al. (2012)