› 2fhd

X-ray diffraction
2.4Å resolution

Crystal structure of Crb2 tandem tudor domains

Released:
DOI: 10.2210/pdb2fhd/pdb
PDB entry 2fhd coloured by chain, front view.
PDB entry 2fhd coloured by chain, side view.
PDB entry 2fhd coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair.
Botuyan MV, Lee J, Ward IM, Kim JE, Thompson JR, Chen J, Mer G
Cell 127 1361-73 (2006)
PMID: 17190600

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160997 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair protein crb2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 153 amino acids
Theoretical weight: 17.61 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: P87074 (Residues: 358-507; Coverage: 19%)
Gene names: SPBC342.05, crb2, rhp9
Sequence domains: DNA repair protein Crb2 Tudor domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 10 x PO4
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 116.924Å b: 116.924Å c: 87.015Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.192 0.256
Expression system: Escherichia coli

Quick links

Citations

246 review citations

Chromatin modifications and their function.
Kouzarides T. (2007)
245 more

4 mentions without citation

Combinatorial readout of dual histone modifications by paired chromatin-associated modules.
Wang et al. (2011)
3 more