› 2fhk

X-ray diffraction
2Å resolution

Crystal structure of formylmethanofuran: tetrahydromethanopterin formyltransferase in complex with its coenzymes

Released:
DOI: 10.2210/pdb2fhk/pdb
PDB entry 2fhk coloured by chain, front view.
PDB entry 2fhk coloured by chain, side view.
PDB entry 2fhk coloured by chain, top view.
Source organism: Methanopyrus kandleri
Primary publication:
The structure of formylmethanofuran: tetrahydromethanopterin formyltransferase in complex with its coenzymes.
Acharya P, Warkentin E, Ermler U, Thauer RK, Shima S
J Mol Biol 357 870-9 (2006)
PMID: 16466742

Function and Biology Details

Reaction catalysed: 
Formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175406 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formylmethanofuran--tetrahydromethanopterin formyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 296 amino acids
Theoretical weight: 31.69 KDa
Source organism: Methanopyrus kandleri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q49610 (Residues: 1-296; Coverage: 100%)
Gene names: MK0116, ftr
Sequence domains:
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand K 18 x K
Ligand MFN 3 x MFN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 86Å b: 74.2Å c: 103.6Å
α: 90° β: 113.6° γ: 90°
R-values:
R R work R free
0.219 0.218 0.245
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

How to make a living by exhaling methane.
Ferry JG. (2010)
2 more