› 2fhl

X-ray diffraction
1.05Å resolution

avidin related protein (AVR4)-BNA complex

Released:
DOI: 10.2210/pdb2fhl/pdb
PDB entry 2fhl coloured by chain, front view.
PDB entry 2fhl coloured by chain, side view.
PDB entry 2fhl coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Factors dictating the pseudocatalytic efficiency of avidins.
Prizant M, Eisenberg-Domovich Y, Hytönen VP, Kulomaa MS, Wilchek M, Bayer EA, Livnah O
J Mol Biol 358 754-63 (2006)
PMID: 16546211

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Avidin-related protein 4/5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 13.7 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P56734 (Residues: 25-145; Coverage: 96%)
Gene names: AVR4, AVR5
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand BNI 2 x BNI
Ligand FMT 10 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41212
Unit cell:
a: 78.039Å b: 78.039Å c: 110.028Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.177
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Brave new (strept)avidins in biotechnology.
Laitinen et al. (2007)
4 other articles

2 mentions without citation

Pre-arrayed Pan-AAV Peptide Display Libraries for Rapid Single-Round Screening.
Börner et al. (2020)
1 more