› 2fj0

X-ray diffraction
2.7Å resolution

Crystal Structure of Juvenile Hormone Esterase from Manduca sexta, with OTFP covalently attached

Released:
DOI: 10.2210/pdb2fj0/pdb
PDB entry 2fj0 coloured by chain, front view.
PDB entry 2fj0 coloured by chain, side view.
PDB entry 2fj0 coloured by chain, top view.
Source organism: Manduca sexta
Primary publication:
Structural studies of a potent insect maturation inhibitor bound to the juvenile hormone esterase of Manduca sexta.
Wogulis M, Wheelock CE, Kamita SG, Hinton AC, Whetstone PA, Hammock BD, Wilson DK
Biochemistry 45 4045-57 (2006)
PMID: 16566578

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190408 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxylic ester hydrolase Chain: A
Molecule details ›
Chain: A
Length: 551 amino acids
Theoretical weight: 62.16 KDa
Source organism: Manduca sexta
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9GPG0 (Residues: 23-573; Coverage: 100%)
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand TFC 1 x TFC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P41212
Unit cell:
a: 96.82Å b: 96.82Å c: 165.395Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.251
Expression system: Trichoplusia ni

Quick links

Citations

2 review citations

Aromatic rings in chemical and biological recognition: energetics and structures.
Salonen et al. (2011)
1 more

9 mentions without citation

Dynamic Roles of Insect Carboxyl/Cholinesterases in Chemical Adaptation.
Cruse et al. (2023)
8 more