› 2fjc

X-ray diffraction
2.5Å resolution

Crystal structure of antigen TpF1 from Treponema pallidum

Released:
DOI: 10.2210/pdb2fjc/pdb
PDB entry 2fjc coloured by chain, front view.
PDB entry 2fjc coloured by chain, side view.
PDB entry 2fjc coloured by chain, top view.
Source organism: Treponema pallidum
Primary publication:
Crystal structure of antigen TpF1 from Treponema pallidum.
Thumiger A, Polenghi A, Papinutto E, Battistutta R, Montecucco C, Zanotti G
Proteins 62 827-30 (2006)
PMID: 16345079

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dodecamer (preferred)
homo 48-mer
Assembly name:
PDBe Complex ID:
PDB-CPX-147788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antigen TpF1 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 156 amino acids
Theoretical weight: 17.25 KDa
Source organism: Treponema pallidum
Expression system: Escherichia coli
UniProt:
  • Canonical: P16665 (Residues: 22-177; Coverage: 88%)
Gene names: TP_1038, tpf1
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

1 bound ligand:
Ligand FE 16 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P321
Unit cell:
a: 184.921Å b: 184.921Å c: 154.883Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.227 0.252
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structure, function and regulation of the DNA-binding protein Dps and its role in acid and oxidative stress resistance in Escherichia coli: a review.
Calhoun et al. (2011)
5 more

3 mentions without citation

Heterologous expression of mycobacterial Esx complexes in Escherichia coli for structural studies is facilitated by the use of maltose binding protein fusions.
Arbing et al. (2013)
2 more