› 2fjt

X-ray diffraction
1.9Å resolution

Adenylyl cyclase class iv from Yersinia pestis

Released:
DOI: 10.2210/pdb2fjt/pdb
PDB entry 2fjt coloured by chain, front view.
PDB entry 2fjt coloured by chain, side view.
PDB entry 2fjt coloured by chain, top view.
Source organism: Yersinia pestis KIM10+
Primary publication:
Structure of the class IV adenylyl cyclase reveals a novel fold.
Gallagher DT, Smith NN, Kim SK, Heroux A, Robinson H, Reddy PT
J Mol Biol 362 114-22 (2006)
PMID: 16905149

Function and Biology Details

Reaction catalysed: 
ATP = 3',5'-cyclic AMP + diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate cyclase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.64 KDa
Source organism: Yersinia pestis KIM10+
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2U2H3Y1 (Residues: 1-179; Coverage: 100%)
Gene names: YPO1178, cyaB
Sequence domains: CYTH domain
Structure domains: Hypothetical Protein Pfu-838710-001

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand CSX 2 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 33.529Å b: 35.548Å c: 71.985Å
α: 88.89° β: 82.63° γ: 65.47°
R-values:
R R work R free
0.216 0.212 0.273
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Biological roles of cAMP: variations on a theme in the different kingdoms of life.
Gancedo JM. (2013)
5 more

6 mentions without citation

The adenylyl cyclase activity of anthrax edema factor.
Tang et al. (2009)
5 more