› 2fk8

X-ray diffraction
2Å resolution

Crystal structure of Hma (MmaA4) from Mycobacterium tuberculosis complexed with S-adenosylmethionine

Released:
DOI: 10.2210/pdb2fk8/pdb
PDB entry 2fk8 coloured by chain, front view.
PDB entry 2fk8 coloured by chain, side view.
PDB entry 2fk8 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Further insight into S-adenosylmethionine-dependent methyltransferases: structural characterization of Hma, an enzyme essential for the biosynthesis of oxygenated mycolic acids in Mycobacterium tuberculosis.
Boissier F, Bardou F, Guillet V, Uttenweiler-Joseph S, Daffé M, Quémard A, Mourey L
J Biol Chem 281 4434-45 (2006)
PMID: 16356931

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181263 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxymycolate synthase MmaA4 Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 36.52 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: Q79FX8 (Residues: 4-301; Coverage: 99%)
Gene names: Rv0642c, hma, mma4, mmaA4
Sequence domains: Mycolic acid cyclopropane synthetase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand SAM 1 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3121
Unit cell:
a: 56.779Å b: 56.779Å c: 199.035Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.179 0.239
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Methylthioadenosine/S-adenosylhomocysteine nucleosidase, a critical enzyme for bacterial metabolism.
Parveen et al. (2011)
7 more

5 mentions without citation

The pathogenic mechanism of Mycobacterium tuberculosis: implication for new drug development.
Yan et al. (2022)
4 more