› 2fko

X-ray diffraction
1.85Å resolution

Structure of PH1591 from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb2fko/pdb
PDB entry 2fko coloured by chain, front view.
PDB entry 2fko coloured by chain, side view.
PDB entry 2fko coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Observation of a calcium-binding site in the gamma-class carbonic anhydrase from Pyrococcus horikoshii.
Jeyakanthan J, Rangarajan S, Mridula P, Kanaujia SP, Shiro Y, Kuramitsu S, Yokoyama S, Sekar K
Acta Crystallogr D Biol Crystallogr 64 1012-9 (2008)
PMID: 18931408

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129841 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
173aa long hypothetical ferripyochelin binding protein Chain: A
Molecule details ›
Chain: A
Length: 173 amino acids
Theoretical weight: 18.94 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59257 (Residues: 1-173; Coverage: 100%)
Gene name: PH1591
Sequence domains: Bacterial transferase hexapeptide (six repeats)
Structure domains: Hexapeptide repeat proteins

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: F4132
Unit cell:
a: 157.412Å b: 157.412Å c: 157.412Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.207
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The gamma class of carbonic anhydrases.
Ferry JG. (2010)
3 more

8 mentions without citation

Neutralizing Antibodies Targeting the Conserved Stem Region of Influenza Hemagglutinin.
Nath Neerukonda et al. (2020)
7 more