› 2fp9

X-ray diffraction
2.96Å resolution

Crystal structure of Native Strictosidine Synthase

Released:
DOI: 10.2210/pdb2fp9/pdb
PDB entry 2fp9 coloured by chain, front view.
PDB entry 2fp9 coloured by chain, side view.
PDB entry 2fp9 coloured by chain, top view.
Source organism: Rauvolfia serpentina
Primary publication:
The structure of Rauvolfia serpentina strictosidine synthase is a novel six-bladed beta-propeller fold in plant proteins.
Ma X, Panjikar S, Koepke J, Loris E, Stöckigt J
Plant Cell 18 907-20 (2006)
PMID: 16531499

Function and Biology Details

Reaction catalysed: 
3-alpha-(S)-strictosidine + H(2)O = tryptamine + secologanin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159353 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Strictosidine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 35.77 KDa
Source organism: Rauvolfia serpentina
Expression system: Escherichia coli
UniProt:
  • Canonical: P68175 (Residues: 23-344; Coverage: 100%)
Gene name: STR1
Sequence domains: Strictosidine synthase
Structure domains: TolB, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand TLA 2 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: R3
Unit cell:
a: 150.276Å b: 150.276Å c: 122.399Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.189 0.237
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Chemistry and biology of monoterpene indole alkaloid biosynthesis.
O'Connor et al. (2006)
15 more

1 mention without citation

Structural analysis of human NHLRC2, mutations of which are associated with FINCA disease.
Biterova et al. (2018)