2fpo

X-ray diffraction
2.05Å resolution

Putative methyltransferase yhhF from Escherichia coli.

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + guanine(966) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(966) in 16S rRNA
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-142386 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase D Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 202 amino acids
Theoretical weight: 22.18 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADX9 (Residues: 1-198; Coverage: 100%)
Gene names: JW3430, b3465, rsmD, yhhF
Sequence domains: Conserved hypothetical protein 95
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 70.504Å b: 96.912Å c: 193.961Å
α: 90° β: 92.21° γ: 90°
R-values:
R R work R free
0.184 0.183 0.217
Expression system: Escherichia coli BL21(DE3)