› 2fr7

X-ray diffraction
2.01Å resolution

Crystal Structure of Cytochrome P450 CYP199A2

Released:
DOI: 10.2210/pdb2fr7/pdb
Model geometry
Fit model/data
PDB entry 2fr7 coloured by chain, front view.
PDB entry 2fr7 coloured by chain, side view.
PDB entry 2fr7 coloured by chain, top view.
Source organism: Rhodopseudomonas palustris CGA009
Primary publication:
Crystal structure of CYP199A2, a para-substituted benzoic acid oxidizing cytochrome P450 from Rhodopseudomonas palustris.
Bell SG, Xu F, Forward I, Bartlam M, Rao Z, Wong LL
J Mol Biol 383 561-74 (2008)
PMID: 18762195

Function and Biology Details

Reaction catalysed: 
4-methoxybenzoate + AH(2) + O(2) = 4-hydroxybenzoate + formaldehyde + A + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179981 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome p450 CYP199A2 Chain: A
Molecule details ›
Chain: A
Length: 412 amino acids
Theoretical weight: 44.67 KDa
Source organism: Rhodopseudomonas palustris CGA009
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6N8N2 (Residues: 1-412; Coverage: 100%)
Gene names: RPA1871, cyp199a2
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 72.16Å b: 74.671Å c: 83.679Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.198 0.23
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Hydrocarbon hydroxylation by cytochrome P450 enzymes.
Ortiz de Montellano PR. (2010)
1 more

5 mentions without citation

Conformational plasticity and structure/function relationships in cytochromes P450.
Pochapsky et al. (2010)
4 more