› 2fsi

X-ray diffraction
2.11Å resolution

Complex SecA:ADP from Escherichia coli

Released:
DOI: 10.2210/pdb2fsi/pdb
PDB entry 2fsi coloured by chain, front view.
PDB entry 2fsi coloured by chain, side view.
PDB entry 2fsi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of dimeric SecA, the Escherichia coli preprotein translocase motor.
Papanikolau Y, Papadovasilaki M, Ravelli RB, McCarthy AA, Cusack S, Economou A, Petratos K
J Mol Biol 366 1545-57 (2007)
PMID: 17229438

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein translocase subunit SecA Chains: A, B
Molecule details ›
Chains: A, B
Length: 853 amino acids
Theoretical weight: 97.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P10408 (Residues: 9-861; Coverage: 95%)
Gene names: JW0096, azi, b0098, pea, prlD, secA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 75.56Å b: 90.43Å c: 163.29Å
α: 90° β: 100.82° γ: 90°
R-values:
R R work R free
0.206 0.203 0.263
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Protein translocation across the bacterial cytoplasmic membrane.
Driessen et al. (2008)
27 more

4 mentions without citation

SecA: a potential antimicrobial target.
Chaudhary et al. (2015)
3 more