› 2fss

X-ray diffraction
1.7Å resolution

Candida boidinii formate dehydrogenase (FDH) K47E mutant

Released:
DOI: 10.2210/pdb2fss/pdb
PDB entry 2fss coloured by chain, front view.
PDB entry 2fss coloured by chain, side view.
PDB entry 2fss coloured by chain, top view.
Source organism: [Candida] boidinii
Primary publication:
High-resolution structures of formate dehydrogenase from Candida boidinii.
Schirwitz K, Schmidt A, Lamzin VS
Protein Sci 16 1146-56 (2007)
PMID: 17525463

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-126747 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 365 amino acids
Theoretical weight: 40.39 KDa
Source organism: [Candida] boidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: O13437 (Residues: 2-364; Coverage: 100%)
Gene names: FDH, FDH1, FDH3
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P1
Unit cell:
a: 53.43Å b: 68.389Å c: 109.369Å
α: 77.93° β: 89.33° γ: 81.34°
R-values:
R R work R free
0.206 0.204 0.249
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Second and Outer Coordination Sphere Effects in Nitrogenase, Hydrogenase, Formate Dehydrogenase, and CO Dehydrogenase.
Stripp et al. (2022)
36 other articles

5 mentions without citation

High-resolution structures of formate dehydrogenase from Candida boidinii.
Schirwitz et al. (2007)
4 more