› 2fuf

X-ray diffraction
1.45Å resolution

Crystal structure of the SV40 large T antigen origin-binding domain

Released:
DOI: 10.2210/pdb2fuf/pdb
PDB entry 2fuf coloured by chain, front view.
PDB entry 2fuf coloured by chain, side view.
PDB entry 2fuf coloured by chain, top view.
Source organism: Macaca mulatta polyomavirus 1
Primary publication:
Crystal structure of the simian virus 40 large T-antigen origin-binding domain.
Meinke G, Bullock PA, Bohm A
J Virol 80 4304-12 (2006)
PMID: 16611889

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large T antigen Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 15.51 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P03070 (Residues: 131-260; Coverage: 18%)
Sequence domains: Origin of replication binding protein
Structure domains: Replication Protein E1; Chain: A,

Ligands and Environments

1 bound ligand:
Ligand FLC 1 x FLC
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P65
Unit cell:
a: 83.669Å b: 83.669Å c: 35.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.169 0.189
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

The Mcm complex: unwinding the mechanism of a replicative helicase.
Bochman et al. (2009)
8 more

16 mentions without citation

The critical protein interactions and structures that elicit growth deregulation in cancer and viral replication.
Ou et al. (2011)
15 more