› 2fvf

Solution NMR

Structure of 10:0-ACP (protein with docked fatty acid)

Released:
DOI: 10.2210/pdb2fvf/pdb
PDB entry 2fvf coloured by chain, ensemble of 20 models, front view.
PDB entry 2fvf coloured by chain, ensemble of 20 models, side view.
PDB entry 2fvf coloured by chain, ensemble of 20 models, top view.
Source organism: Spinacia oleracea
Primary publication:
Solution structures of spinach acyl carrier protein with decanoate and stearate.
Zornetzer GA, Fox BG, Markley JL
Biochemistry 45 5217-27 (2006)
PMID: 16618110

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139792 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl carrier protein 1, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 82 amino acids
Theoretical weight: 8.84 KDa
Source organism: Spinacia oleracea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07854 (Residues: 57-138; Coverage: 59%)
Gene name: ACL1.1
Sequence domains: Phosphopantetheine attachment site
Structure domains: ACP-like

Ligands and Environments

2 bound ligands:
Ligand PNS 1 x PNS
Ligand DKA 1 x DKA
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Initial structure from torsion angle dynamics (in CYANA). Fatty acid and phosphopantetheine were added and docked using ambiguous distance restraints in XPLOR-NIH
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Current understanding of fatty acid biosynthesis and the acyl carrier protein.
Chan et al. (2010)
12 more

5 mentions without citation

Solution structures of spinach acyl carrier protein with decanoate and stearate.
Zornetzer et al. (2006)
4 more