2fwi

X-ray diffraction
1.94Å resolution

Structure of PurE (N5-carboxyaminoimidazole ribonucleotide mutase) H59D, from the acidophilic bacterium Acetobacter aceti, complexed with 5-aminoimidazole ribonucleotide (AIR)

Released:

Function and Biology Details

Reaction catalysed:
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
PDBe Complex ID:
PDB-CPX-173882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N5-carboxyaminoimidazole ribonucleotide mutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 18.86 KDa
Source organism: Acetobacter aceti
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2QJL3 (Residues: 1-182; Coverage: 100%)
Gene name: purE
Sequence domains: AIR carboxylase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I422
Unit cell:
a: 99.046Å b: 99.046Å c: 163.829Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.191
Expression system: Escherichia coli