› 2fxl

X-ray diffraction
1.76Å resolution

Urate oxidase from aspergillus flavus complexed with allantoin

Released:
DOI: 10.2210/pdb2fxl/pdb
PDB entry 2fxl coloured by chain, front view.
PDB entry 2fxl coloured by chain, side view.
PDB entry 2fxl coloured by chain, top view.
Source organism: Aspergillus flavus
Primary publication:
Recapture of [S]-allantoin, the product of the two-step degradation of uric acid, by urate oxidase.
Gabison L, Chiadmi M, Colloc'h N, Castro B, El Hajji M, Prangé T
FEBS Lett 580 2087-91 (2006)
PMID: 16545381

Function and Biology Details

Reaction catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uricase Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 34.2 KDa
Source organism: Aspergillus flavus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q00511 (Residues: 2-302; Coverage: 100%)
Gene names: Uox, uaZ
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

1 bound ligand:
Ligand 2AL 1 x 2AL
1 modified residue:
Ligand SAC 1 x SAC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I222
Unit cell:
a: 80.364Å b: 96.086Å c: 105.368Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 not available not available
Expression system: Saccharomyces cerevisiae

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Citations

1 review citation

Large crystal growth by thermal control allows combined X-ray and neutron crystallographic studies to elucidate the protonation states in Aspergillus flavus urate oxidase.
Oksanen et al. (2009)
13 other articles

2 mentions without citation

The Recognition of Identical Ligands by Unrelated Proteins.
Barelier et al. (2015)
1 more