› 2fxt

X-ray diffraction
3.2Å resolution

Crystal Structure of Yeast Tim44

Released:
DOI: 10.2210/pdb2fxt/pdb
PDB entry 2fxt coloured by chain, front view.
PDB entry 2fxt coloured by chain, side view.
PDB entry 2fxt coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of yeast mitochondrial peripheral membrane protein Tim44p C-terminal domain.
Josyula R, Jin Z, Fu Z, Sha B
J Mol Biol 359 798-804 (2006)
PMID: 16647716

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial import inner membrane translocase subunit TIM44 Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 22.02 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q01852 (Residues: 234-425; Coverage: 45%)
Gene names: ISP45, MIM44, MPI1, TIM44, YIL022W
Sequence domains: Tim44-like domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6322
Unit cell:
a: 124.25Å b: 124.25Å c: 77.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.316 0.316 0.368
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Translocation of proteins into mitochondria.
Neupert et al. (2007)
6 more

5 mentions without citation

Hsp70 at the membrane: driving protein translocation.
Craig EA. (2018)
4 more