› 2fyh

Solution NMR

Solution structure of the 2'-5' RNA ligase-like protein from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb2fyh/pdb
PDB entry 2fyh coloured by chain, ensemble of 20 models, front view.
PDB entry 2fyh coloured by chain, ensemble of 20 models, side view.
PDB entry 2fyh coloured by chain, ensemble of 20 models, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermophilic archaeon, Pyrococcus furiosus.
Kanai A, Sato A, Fukuda Y, Okada K, Matsuda T, Sakamoto T, Muto Y, Yokoyama S, Kawai G, Tomita M
RNA 15 420-31 (2009)
PMID: 19155324

Function and Biology Details

Reaction catalysed: 
(Ribonucleotide)(n)-2',3'-cyclic phosphate + H(2)O = (ribonucleotide)(n)-2'-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186503 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA 2',3'-cyclic phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.86 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U4Q3 (Residues: 1-184; Coverage: 100%)
Gene name: PF0027
Sequence domains: LigT like Phosphoesterase
Structure domains: Cyclic phosphodiesterase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics, simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Diversity and roles of (t)RNA ligases.
Popow et al. (2012)
12 other articles

2 mentions without citation

Myelin 2',3'-cyclic nucleotide 3'-phosphodiesterase: active-site ligand binding and molecular conformation.
Myllykoski et al. (2012)
1 more