› 2fyn

X-ray diffraction
3.2Å resolution

Crystal Structure Analysis of the double mutant Rhodobacter Sphaeroides bc1 complex

Released:
DOI: 10.2210/pdb2fyn/pdb
PDB entry 2fyn coloured by chain, front view.
PDB entry 2fyn coloured by chain, side view.
PDB entry 2fyn coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Surface-modulated motion switch: capture and release of iron-sulfur protein in the cytochrome bc1 complex.
Esser L, Gong X, Yang S, Yu L, Yu CA, Xia D
Proc Natl Acad Sci U S A 103 13045-50 (2006)
PMID: 16924113

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-169747 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytochrome b Chains: A, D, G, J, M, P
Molecule details ›
Chains: A, D, G, J, M, P
Length: 445 amino acids
Theoretical weight: 50.16 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: Q02761 (Residues: 1-445; Coverage: 100%)
Gene names: fbcB, petB
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome c1 Chains: B, E, H, K, N, Q
Molecule details ›
Chains: B, E, H, K, N, Q
Length: 269 amino acids
Theoretical weight: 29.37 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: Q02760 (Residues: 23-285; Coverage: 100%)
Gene names: fbcC, petC
Sequence domains: Cytochrome C1 family
Structure domains:
Ubiquinol-cytochrome c reductase iron-sulfur subunit Chains: C, F, I, L, O, R
Molecule details ›
Chains: C, F, I, L, O, R
Length: 187 amino acids
Theoretical weight: 19.92 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: Q02762 (Residues: 1-187; Coverage: 100%)
Gene names: fbcF, petA
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand HEM 18 x HEM
Ligand SMA 6 x SMA
Ligand LOP 6 x LOP
Ligand FES 6 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 351.3Å b: 147.13Å c: 160.83Å
α: 90° β: 103.94° γ: 90°
R-values:
R R work R free
0.224 0.224 0.254
Expression system: Cereibacter sphaeroides

Quick links

Citations

17 review citations

The Q-cycle reviewed: How well does a monomeric mechanism of the bc(1) complex account for the function of a dimeric complex?
Crofts et al. (2008)
16 more

17 mentions without citation

Structural analysis of cytochrome bc1 complexes: implications to the mechanism of function.
Xia et al. (2013)
16 more