› 2fyv

X-ray diffraction
1.9Å resolution

Golgi alpha-mannosidase II complex with an amino-salacinol carboxylate analog

Released:
DOI: 10.2210/pdb2fyv/pdb
PDB entry 2fyv coloured by chain, front view.
PDB entry 2fyv coloured by chain, side view.
PDB entry 2fyv coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Synthesis, enzymatic activity, and X-ray crystallography of an unusual class of amino acids.
Chen W, Kuntz DA, Hamlet T, Sim L, Rose DR, Mario Pinto B
Bioorg Med Chem 14 8332-40 (2006)
PMID: 17010621

Function and Biology Details

Reaction catalysed: 
Man(5)GlcNAc(3)-[protein] + 2 H(2)O = Man(3)GlcNAc(3)-[protein] + 2 alpha-D-mannopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

5 bound ligands:
Ligand NAG 1 x NAG
Ligand PO4 1 x PO4
Ligand ZN 1 x ZN
Ligand W72 1 x W72
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 68.806Å b: 108.635Å c: 137.384Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.162 0.212
Expression system: Drosophila melanogaster

Quick links

Citations

2 review citations

Zwitterionic glycosidase inhibitors: salacinol and related analogues.
Mohan et al. (2007)
1 more

2 mentions without citation

Structure based virtual screening of natural product molecules as glycosidase inhibitors.
Moorthy et al. (2021)
1 more