› 2fzv

X-ray diffraction
1.7Å resolution

Crystal Structure of an apo form of a Flavin-binding Protein from Shigella flexneri

Released:
DOI: 10.2210/pdb2fzv/pdb
PDB entry 2fzv coloured by chain, front view.
PDB entry 2fzv coloured by chain, side view.
PDB entry 2fzv coloured by chain, top view.
Source organism: Shigella flexneri 2a str. 2457T
Primary publication:
Crystal structure of an apo form of Shigella flexneri ArsH protein with an NADPH-dependent FMN reductase activity.
Vorontsov II, Minasov G, Brunzelle JS, Shuvalova L, Kiryukhina O, Collart FR, Anderson WF
Protein Sci 16 2483-90 (2007)
PMID: 17962405

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-182040 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH-dependent FMN reductase ArsH Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 279 amino acids
Theoretical weight: 31.18 KDa
Source organism: Shigella flexneri 2a str. 2457T
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7UC03 (Residues: 1-255; Coverage: 100%)
Gene names: S2709, SF2457T_0606, arsH
Sequence domains: NADPH-dependent FMN reductase
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P43212
Unit cell:
a: 117.821Å b: 117.821Å c: 154.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Microbial responses to environmental arsenic.
Páez-Espino et al. (2009)
2 more

3 mentions without citation

Crystal structures of NADH:FMN oxidoreductase (EmoB) at different stages of catalysis.
Nissen et al. (2008)
2 more