› 2g0b

X-ray diffraction
3Å resolution

The structure of FeeM, an N-acyl amino acid synthase from uncultured soil microbes

Released:
DOI: 10.2210/pdb2g0b/pdb
PDB entry 2g0b coloured by chain, front view.
PDB entry 2g0b coloured by chain, side view.
PDB entry 2g0b coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding.
Van Wagoner RM, Clardy J
Structure 14 1425-35 (2006)
PMID: 16962973

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-185197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Long-chain N-acyl amino acid synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 198 amino acids
Theoretical weight: 21.78 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KNZ7 (Residues: 1-196; Coverage: 100%)
Structure domains: Aminopeptidase

Ligands and Environments

1 bound ligand:
Ligand NLT 8 x NLT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: I4122
Unit cell:
a: 182.832Å b: 182.832Å c: 287.429Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.255 0.292
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Structural basis of acyl-homoserine lactone-dependent signaling.
Churchill et al. (2011)
3 more

3 mentions without citation

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria.
Kumar et al. (2022)
2 more