› 2g0c

X-ray diffraction
1.7Å resolution

Structure of the RNA binding domain (residues 404-479) of the Bacillus subtilis YxiN protein

Released:
DOI: 10.2210/pdb2g0c/pdb
PDB entry 2g0c coloured by chain, front view.
PDB entry 2g0c coloured by chain, side view.
PDB entry 2g0c coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The domain of the Bacillus subtilis DEAD-box helicase YxiN that is responsible for specific binding of 23S rRNA has an RNA recognition motif fold.
Wang S, Hu Y, Overgaard MT, Karginov FV, Uhlenbeck OC, McKay DB
RNA 12 959-67 (2006)
PMID: 16611943

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-154603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase DbpA Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.29 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42305 (Residues: 404-479; Coverage: 16%)
Gene names: BSU39110, SS8E, dbpA, deaD, yxiN
Sequence domains: DbpA RNA binding domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P41212
Unit cell:
a: 51.01Å b: 51.01Å c: 64.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.253 0.276
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Dead-box proteins: a family affair--active and passive players in RNP-remodeling.
Linder P. (2006)
14 more

5 mentions without citation

Structure of the RNA binding domain of a DEAD-box helicase bound to its ribosomal RNA target reveals a novel mode of recognition by an RNA recognition motif.
Hardin et al. (2010)
4 more