› 2g1j

X-ray diffraction
2Å resolution

Crystal structure of Mycobacterium tuberculosis Shikimate Kinase at 2.0 angstrom resolution

Released:
DOI: 10.2210/pdb2g1j/pdb
PDB entry 2g1j coloured by chain, front view.
PDB entry 2g1j coloured by chain, side view.
PDB entry 2g1j coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of Mycobacterium tuberculosis shikimate kinase in complex with shikimic acid and an ATP analogue.
Gan J, Gu Y, Li Y, Yan H, Ji X
Biochemistry 45 8539-45 (2006)
PMID: 16834327

Function and Biology Details

Reaction catalysed: 
ATP + shikimate = ADP + shikimate 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162025 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shikimate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 176 amino acids
Theoretical weight: 18.61 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPY3 (Residues: 1-176; Coverage: 100%)
Gene names: MTCY159.17, Rv2539c, aroK
Sequence domains: Shikimate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P31
Unit cell:
a: 90.222Å b: 90.222Å c: 41.356Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.175 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The shikimate pathway and aromatic amino Acid biosynthesis in plants.
Maeda et al. (2012)
23 other articles

1 mention without citation

Selective Mycobacterium tuberculosis Shikimate Kinase Inhibitors as Potential Antibacterials.
Gordon et al. (2015)