› 2g2d

X-ray diffraction
2Å resolution

Crystal structure of a putative pduO-type ATP:cobalamin adenosyltransferase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb2g2d/pdb
PDB entry 2g2d coloured by chain, front view.
PDB entry 2g2d coloured by chain, side view.
PDB entry 2g2d coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Moon JH, Kaviratne A, Yu M, Bursey EH, Hung L-W, Lekin TP, Segelke BW, Terwilliger TC, Kim C-Y, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Reaction catalysed: 
(1a) 2 cob(II)alamin + 2 [corrinoid adenosyltransferase] = 2 [corrinoid adenosyltransferase]-cob(II)alamin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Corrinoid adenosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 20.72 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WP99 (Residues: 1-193; Coverage: 100%)
Gene names: MTCY373.34c, Rv1314c
Sequence domains: Cobalamin adenosyltransferase
Structure domains: Cobalamin adenosyltransferase-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: I4132
Unit cell:
a: 131.318Å b: 131.318Å c: 131.318Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.194 0.228
Expression system: Escherichia coli BL21(DE3)

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