› 2g2n

X-ray diffraction
1.65Å resolution

Crystal Structure of E.coli transthyretin-related protein with bound Zn

Released:
DOI: 10.2210/pdb2g2n/pdb
PDB entry 2g2n coloured by chain, front view.
PDB entry 2g2n coloured by chain, side view.
PDB entry 2g2n coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The transthyretin-related protein: structural investigation of a novel protein family.
Lundberg E, Bäckström S, Sauer UH, Sauer-Eriksson AE
J Struct Biol 155 445-57 (2006)
PMID: 16723258

Function and Biology Details

Reaction catalysed: 
5-hydroxyisourate + H(2)O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160079 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-hydroxyisourate hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 114 amino acids
Theoretical weight: 13.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P76341 (Residues: 24-137; Coverage: 100%)
Gene names: JW1953, b1970, hiuH, yedX
Sequence domains: HIUase/Transthyretin family
Structure domains: Transthyretin/hydroxyisourate hydrolase domain

Ligands and Environments

2 bound ligands:
Ligand ZN 18 x ZN
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P21
Unit cell:
a: 44.97Å b: 92.67Å c: 58.05Å
α: 90° β: 103.86° γ: 90°
R-values:
R R work R free
0.2 0.198 0.234
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure of zebra fish HIUase: insights into evolution of an enzyme to a hormone transporter.
Zanotti et al. (2006)
3 more

1 mention without citation

Structural and kinetic insights into the mechanism of 5-hydroxyisourate hydrolase from Klebsiella pneumoniae.
French et al. (2011)