› 2g4b

X-ray diffraction
2.5Å resolution

Structure of U2AF65 variant with polyuridine tract

Released:
DOI: 10.2210/pdb2g4b/pdb
PDB entry 2g4b coloured by chain, front view.
PDB entry 2g4b coloured by chain, side view.
PDB entry 2g4b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65.
Sickmier EA, Frato KE, Shen H, Paranawithana SR, Green MR, Kielkopf CL
Mol Cell 23 49-59 (2006)
PMID: 16818232

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150726 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 172 amino acids
Theoretical weight: 18.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26368 (Residues: 147-336; Coverage: 36%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
5'-R(P*UP*UP*UP*UP*UP*UP*U)-3' Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.1 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand DIO 1 x DIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P6522
Unit cell:
a: 129.388Å b: 129.388Å c: 62.645Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.306 0.261 0.276
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

24 review citations

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
23 more

19 mentions without citation

The structure of human cleavage factor I(m) hints at functions beyond UGUA-specific RNA binding: a role in alternative polyadenylation and a potential link to 5' capping and splicing.
Yang et al. (2011)
18 more